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American Association for Cancer Research

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miR-137 Regulates the Tumorigenicity of Colon Cancer Stem Cells through the Inhibition of DCLK1

Overview of attention for article published in Molecular Cancer Research, April 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (87th percentile)
  • High Attention Score compared to outputs of the same age and source (99th percentile)

Mentioned by

news
1 news outlet
twitter
4 tweeters

Citations

dimensions_citation
70 Dimensions

Readers on

mendeley
43 Mendeley
Title
miR-137 Regulates the Tumorigenicity of Colon Cancer Stem Cells through the Inhibition of DCLK1
Published in
Molecular Cancer Research, April 2016
DOI 10.1158/1541-7786.mcr-15-0380
Pubmed ID
Authors

Masazumi Sakaguchi, Shigeo Hisamori, Nobu Oshima, Fumiaki Sato, Yohei Shimono, Yoshiharu Sakai

Abstract

MicroRNAs (miRNAs) have important roles in regulating cancer stem cell (CSC) properties and are considered to be potential therapeutic targets. However, few studies have focused on miRNAs which are specifically related to colon CSCs. Here a PCR-based miRNA profiling analysis of normal colon stem cell (NCSCs) and colon CSCs (EpCAM+/CD44+/CD66a-) identified miRNAs which regulate colon CSC properties. Interestingly, miRNA-137 (miR-137) expression was downregulated in the colon CSCs compared to NCSCs, while doublecortin-like kinase 1 (DCLK1) mRNA was highly expressed in the colon CSCs but low in the NCSCs. In fact, DCLK1-positive cancer cells were widely distributed in clinically resected colon cancer specimens, while DCLK1-positve epithelial cells were rarely detected in normal colon tissues including the crypt bottoms. Luciferase assay and immunoblot analysis revealed that miR-137 regulated DCLK1 gene expression. Transduction of exogenous miR-137 suppressed the development of colon cancer organoids in vitro and the tumorigenecity of colon cancer cells in vivo without affecting the growth of normal intestinal organoids. Furthermore, the suppression of miR-137 enhanced the organoid development of normal colon cells. These data demonstrate that miR-137 has the capacity to suppress the tumorigenicity of colon CSCs and that maintained expression of miR-137 in NCSCs contributes to suppressing uncontrolled cell proliferation through the inhibition of DCLK1 expression. The miR-137/DCLK1 axis as an important regulator in NCSCs and colon CSCs; further understanding of this axis may foster the development of potential gene therapeutic strategies targeting colon CSCs.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Iran, Islamic Republic of 1 2%
Unknown 42 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 19%
Researcher 7 16%
Student > Master 7 16%
Student > Bachelor 5 12%
Professor > Associate Professor 3 7%
Other 5 12%
Unknown 8 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 28%
Biochemistry, Genetics and Molecular Biology 11 26%
Medicine and Dentistry 7 16%
Nursing and Health Professions 1 2%
Computer Science 1 2%
Other 3 7%
Unknown 8 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 January 2022.
All research outputs
#2,423,077
of 21,569,681 outputs
Outputs from Molecular Cancer Research
#122
of 1,822 outputs
Outputs of similar age
#49,008
of 404,567 outputs
Outputs of similar age from Molecular Cancer Research
#1
of 30 outputs
Altmetric has tracked 21,569,681 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,822 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.9. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 404,567 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 30 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.